AICR Fellow / Non-clinical Lecturer, Medical Genetics
Cardiff University, United Kingdom (Wales)
Period of Award
1st October 2006 – 30th September 2013
Identifying and characterising novel mammalian target of rapamycin (mTOR) substrates
Cells have a complex internal control mechanism, made up of numerous interacting proteins and genes, that regulate how the cells behave, how fast they divide, etc. This control mechanism is operating differently in cancer cells, as they behave very differently from normal cells, multiplying much faster and spreading into surrounding tissues. Several years ago, a protein called mTOR was discovered to play a central role in this internal control mechanism. Although it was discovered that mTOR could chemically modify other proteins, it was involved in controlling so many different aspects of cell activity that it proved very difficult to define how it worked. Dr Tee will use a number of new techniques to identify those proteins which interact directly with mTOR. He will then find out which ones are chemically modified by mTOR. These will be the proteins in the control mechanism that mTOR controls directly. Once they have been identified, he will be able to find out what cell activities they are involved with and how they, in turn, control them.
Aims and Outcomes So Far
Aims: (1) Uncover novel downstream signalling targets from the mTOR/raptor complex.
(2) Characterise novel targets through raptor interaction and mapping mTOR-dependent protein phosphorylation. Keywords
Stephen C. Land and Andrews R. Tee. Hypoxia-inducible Factor 1α Is Regulated by the Mammalian Target of Rapamycin (mTOR) via an mTOR Signaling Motif. Journal of Biological Chemistry, 282 (28). pp. 20534-20543.
Watch a lab tour with Dr Tee here.